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Bio-Techne corporation
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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: Translocation of Full-length Bid to Mitochondria during Anoikis
doi: 10.1074/jbc.m313375200
Figure Lengend Snippet: FIG. 1. Full-length Bid translocates to the membrane fraction of epithelial cells during anoikis. a, FSK-7 cells in monolayer cul- ture were either left attached (Adh) or detached and maintained on poly-HEMA for 15 min, 1 h, or 4 h. The cells were separated into soluble (sol) and membrane (mem) fractions. The samples were separated by SDS-PAGE and immunoblotted for Bid, APAF-1, mitochondrial Hsp70 (mtHsp70), or active caspase 3. b, fractions as in a were immunoblotted for Bax, Bcl-XL, Bak, and Bad. c, samples prepared as in a were immunoblotted for Bid. The samples were run along side 1 ng of recom- binant tBid. tBid was only just detectable in the membrane fraction of detached cells after prolonged exposure of the immunoblot (bottom panel).
Article Snippet: Reagents—Polyclonal goat anti-Bid, rabbit anti-Bad, and
Techniques: Membrane, SDS Page, Western Blot